The paper presents a comparison of the contents of macroelements: N, P, K, Ca and Mg in the biomass of the maize grown for grain, taking into account the developmental phases, the varieties and the method of fertilization. The content of mineral elements in maize changed significantly depending on the developmental phase. The highest content of mineral elements was shown in the phase of 8 leaves, whereas the smallest was found in the phases of straw (N, P, K) and in grain (Mg, Ca) maturity. The variety factor affected the content of nitrogen and calcium. Variety ‘Ravello’ flint was characteristic of the highest content of nitrogen, while the highest level of calcium was determined in variety ‘Oldman’ dent. Row fertilization considerably decreased only the content of nitrogen and increased the content of phosphorus in maize in comparison to broadcast fertilization. The highest uptake of nitrogen, phosphorus and potassium were noted with the yield of the grain, while that of magnesium and calcium in the phase of tassel formation (BBCH 50) of maize. The variety factor affected significantly the uptake of nitrogen, phosphorus, potassium and magnesium by maize. The highest uptake of macroelements was characteristic of ‘Ravello’ flint. Row fertilization caused a significant increase in the uptake of all macroelements by maize in relation to broadcast fertilization.