The objective of the presented studies was the determination of the amounts of cadmium and lead passing into the edible part of tomato fruit and comparison of these metals in the fruits of the first and the third bunch of tomato. Tomato plants of ‘Recento’ cultivar were grown in 10 dm3 containers, in horticultural substrates with an addition of different organic substrates. The above substrates were supplemented with the studied metals in the following amounts: cadmium – 0, 2.5, 10 mg dm-3 and lead – 0, 25, 100 mg dm-3. The experimental studies showed significant differences in Cd and Pb contents in the fruits of the first and the third tomato bunch and in fruits harvested from plants grown on different horticultural substrates. The least amounts of the metals were found in the fruits of the third bunch of tomato and in the fruits of plants grown on substrates with an addition of brown coal.